Derivative Assay:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Migration:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Staining:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Expressing:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Transformation Assay:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Binding Assay:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Luciferase:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Activity Assay:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Transfection:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Immunofluorescence:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Biomarker Discovery:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Standard Deviation:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Gene Expression:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Quantitative Proteomics:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Immunohistochemistry:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Functional Assay:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
In Vitro:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Western Blot:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Quantitative RT-PCR:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Knockdown:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
CCK-8 Assay:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Microarray:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Marker:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Reporter Assay:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Plasmid Preparation:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Control:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
CRISPR:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Stable Transfection:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Transwell Assay:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
In Vivo:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Imaging:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Injection:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Double Staining:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Flow Cytometry:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
RNA Sequencing:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Negative Control:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
TUNEL Assay:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Modification:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Co-Immunoprecipitation Assay:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Pull Down Assay:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Over Expression:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Real-time Polymerase Chain Reaction:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
In Situ Hybridization:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Cell Cycle Assay:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Inhibition:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
Invasion Assay:Article Title: Lactylome analyses suggest systematic lysine- lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia
Article Snippet: All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).All participants provided informed consent before enrollment, and the study was approved by the Institutional Research Ethics Committee of the Hospital of Stomatology, Sun Yat-Sen University (KQEC-2020-16-03).. Human OSCC cell lines SCC25, HSC3, CAL33 and HN6 was purchased from the American Type Culture Collection.. Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).Normal oral keratinocyte (NOK) was provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA).
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Article Snippet: Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.Cell lines and Culture HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, International Journal of Oral Science (2022) 14:36 USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN
Article Snippet: HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors.. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US).. Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells.
Article Title: Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Article Snippet: All patients signed an informed consent form for participation in this study.All patients signed an informed consent form for participation in this study.. The human OSCC cell lines SCC25 and CAL27 were obtained from the American Type Culture Collection (ATCC).. SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).SCC25 cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM/F12, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, WISENT, Canada) and 400 ng/mL hydrocortisone (H811182, MACKLIN, China).
Article Title: Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress‐Induced Apoptosis via Promoting MRE11‐RAD50‐NBS1 Complex Formation
Article Snippet: Human OSCC cell lines SCC25 and CAL27 were purchased from American Type Culture Collection.. Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan University, China).Other cell lines including HEK293 (human embryonic kidney cell line), Hela (human cervical cancer cell line), PANC‐1 (human pancreatic cancer cell line), NOK (human normal oral keratinocytes), DOK (human dysplastic oral keratinocytes), and other human OSCC cell lines (SAS, UM‐1, UM‐2, SCC15, HSC‐2, and HSC‐3) were all obtained from State Key Laboratory of Oral Diseases (Sichuan..
Article Title: lncRNA NR4A1AS Upregulates miR-221 Through Demethylation to Promote Cell Proliferation in Oral Squamous Cell Carcinoma
Article Snippet: Human OSCC cell lines SCC25 and SCC090 were purchased from ATCC (USA) and used in the following experiments.. According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.According to the instructions from ATCC, cells were cultivated in a medium composed of 90% Eagle’s Minimum Essential Medium and 10% fetal bovine serum (FBS) supplemented with 2 mM L-Glutamine.
Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway
Article Snippet: The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).The human OSCC cell line UM1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA), and HSC-3 (OSCC) and normal oral keratinocyte (NOK) cell lines were kindly provided by J. Silvio Gutkind (National Institutes of Health, Bethesda, MD, USA).. The human OSCC cell lines SCC25 and CAL27 were obtained from ATCC (Rockville, MD, USA).. The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).The HEK 293 cell line was purchased from the National Centre for Cell Science (NCCS; Pune, India).
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